Marked Underestimation of Serum 25-hydroxyvitamin D Concentrations by The Abbot Architect Chemiluminescent Microparticle Immunoassay in Patients Receiving Vitamin D2 Supplementation

  • Nipith Charoenngam
    Affiliations
    Section Endocrinology, Diabetes, Nutrition and Weight Management, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts
    Department of Medicine, Mount Auburn Hospital, Harvard Medical School, Cambridge, Massachusetts
    Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
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  • Michael F. Holick
    Affiliations
    Section Endocrinology, Diabetes, Nutrition and Weight Management, Department of Medicine, Boston University School of Medicine, Boston, MA 02118
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Published:October 14, 2021DOI:https://doi.org/10.1016/j.eprac.2021.10.002
      There are several methods of measurement for serum 25-hydroxyvitamin D [25(OH)D] concentration, including the gold standard liquid chromatography tandem mass spectrometry (LC-MS/MS), as well as immunoassays and high-performance liquid chromatography. Some variability in the detectability of 25(OH)D2 and 25(OH)D3 across the methods has been noted.
      • Garnett E.
      • Li J.
      • Rajapakshe D.
      • Tam E.
      • Meng Q.H.
      • Devaraj S.
      Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3.
      • Glendenning P.
      • Taranto M.
      • Noble J.M.
      • et al.
      Current assays overestimate 25-hydroxyvitamin D3 and underestimate 25-hydroxyvitamin D2 compared with HPLC: need for assay-specific decision limits and metabolite-specific assays.
      • Freeman J.
      • Wilson K.
      • Spears R.
      • Shalhoub V.
      • Sibley P.
      Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2.
      • Li L.
      • Zeng Q.
      • Yuan J.
      • Xie Z.
      Performance evaluation of two immunoassays for 25-hydroxyvitamin D.
      • Cavalier E.
      • Lukas P.
      • Bekaert A.C.
      • et al.
      Analytical and clinical validation of the new Abbot Architect 25(OH)D assay: fit for purpose?.
      The most frequently used assays in general clinical practice are automated immunoassays, owing to their affordability.
      • Dirks N.F.
      • Ackermans M.T.
      • Lips P.
      • et al.
      The when, what & how of measuring vitamin D metabolism in clinical medicine.
      However, limited data from clinical and experimental studies have raised concerns over the accuracy of automated immunoassays in evaluating vitamin D status in patients who receive vitamin D2 supplementation.
      • Garnett E.
      • Li J.
      • Rajapakshe D.
      • Tam E.
      • Meng Q.H.
      • Devaraj S.
      Efficacy of two vitamin D immunoassays to detect 25-OH vitamin D2 and D3.
      ,
      • Li L.
      • Zeng Q.
      • Yuan J.
      • Xie Z.
      Performance evaluation of two immunoassays for 25-hydroxyvitamin D.
      ,
      • Charoenngam N.
      • Shirvani A.
      • Holick M.F.
      Vitamin D for skeletal and non-skeletal health: what we should know.

      Abbreviations:

      25(OH)D (25-hydroxyvitamin D), LC-MS/MS (liquid chromatography tandem mass spectrometry)
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